Structure-mechanics relationships of collagen fibrils in the osteogenesis imperfecta mouse model
OG Andriotis and SW Chang and M Vanleene and PH Howarth and DE Davies and SJ Shefelbine and MJ Buehler and PJ Thurner, JOURNAL OF THE ROYAL SOCIETY INTERFACE, 12, 20150701 (2015).
The collagen molecule, which is the building block of collagen fibrils, is a triple helix of two alpha 1(I) chains and one alpha 2(I) chain. However, in the severe mouse model of osteogenesis imperfecta (OIM), deletion of the COL1A2 gene results in the substitution of the alpha 2(I) chain by one alpha 1(I) chain. As this substitution severely impairs the structure and mechanics of collagen-rich tissues at the tissue and organ level, the main aim of this study was to investigate how the structure and mechanics are altered in OIM collagen fibrils. Comparing results from atomic force microscopy imaging and cantilever- based nanoindentation on collagen fibrils from OIM and wild-type (WT) animals, we found a 33% lower indentation modulus in OIM when air-dried (bound water present) and an almost fivefold higher indentation modulus in OIM collagen fibrils when fully hydrated (bound and unbound water present) in phosphate-buffered saline solution (PBS) compared with WT collagen fibrils. These mechanical changes were accompanied by an impaired swelling upon hydration within PBS. Our experimental and atomistic simulation results show how the structure and mechanics are altered at the individual collagen fibril level as a result of collagen gene mutation in OIM. We envisage that the combination of experimental and modelling approaches could allow mechanical phenotyping at the collagen fibril level of virtually any alteration of collagen structure or chemistry.
Return to Publications page